Journal papers
Li-Hao Yeh; Shwetadwip Chowdhury; Laura Waller
Computational structured illumination for high-content fluorescence and phase microscopy Journal Article
In: Biomedical optics express, vol. 10, no. 4, pp. 1978–1998, 2019.
Abstract | Links | BibTeX | Tags: diffuser, fluorescence imaging, high-throughput, phase imaging, structured illumination, super-resolution
@article{yeh2019computational,
title = {Computational structured illumination for high-content fluorescence and phase microscopy},
author = { Li-Hao Yeh and Shwetadwip Chowdhury and Laura Waller},
url = {https://www.osapublishing.org/boe/abstract.cfm?uri=boe-10-4-1978
https://doi.org/10.1364/BOE.10.001978},
doi = {10.1364/BOE.10.001978},
year = {2019},
date = {2019-04-01},
journal = {Biomedical optics express},
volume = {10},
number = {4},
pages = {1978--1998},
publisher = {Optical Society of America},
abstract = {High-content biological microscopy targets high-resolution imaging across large fields-of-view (FOVs). Recent works have demonstrated that computational imaging can provide efficient solutions for high-content microscopy. Here, we use speckle structured illumination microscopy (SIM) as a robust and cost-effective solution for high-content fluorescence microscopy with simultaneous high-content quantitative phase (QP). This multi-modal compatibility is essential for studies requiring cross-correlative biological analysis. Our method uses laterally-translated Scotch tape to generate high-resolution speckle illumination patterns across a large FOV. Custom optimization algorithms then jointly reconstruct the sample’s super-resolution fluorescent (incoherent) and QP (coherent) distributions, while digitally correcting for system imperfections such as unknown speckle illumination patterns, system aberrations and pattern translations. Beyond previous linear SIM works, we achieve resolution gains of 4× the objective’s diffraction-limited native resolution, resulting in 700 nm fluorescence and 1.2 μm QP resolution, across a FOV of 2×2.7 mm 2, giving a space-bandwidth product (SBP) of 60 megapixels.},
keywords = {diffuser, fluorescence imaging, high-throughput, phase imaging, structured illumination, super-resolution},
pubstate = {published},
tppubtype = {article}
}
High-content biological microscopy targets high-resolution imaging across large fields-of-view (FOVs). Recent works have demonstrated that computational imaging can provide efficient solutions for high-content microscopy. Here, we use speckle structured illumination microscopy (SIM) as a robust and cost-effective solution for high-content fluorescence microscopy with simultaneous high-content quantitative phase (QP). This multi-modal compatibility is essential for studies requiring cross-correlative biological analysis. Our method uses laterally-translated Scotch tape to generate high-resolution speckle illumination patterns across a large FOV. Custom optimization algorithms then jointly reconstruct the sample’s super-resolution fluorescent (incoherent) and QP (coherent) distributions, while digitally correcting for system imperfections such as unknown speckle illumination patterns, system aberrations and pattern translations. Beyond previous linear SIM works, we achieve resolution gains of 4× the objective’s diffraction-limited native resolution, resulting in 700 nm fluorescence and 1.2 μm QP resolution, across a FOV of 2×2.7 mm 2, giving a space-bandwidth product (SBP) of 60 megapixels.